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Analysis of the experiments conducted in the study of micro biology

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Shaharu B.
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  1. Definition
  2. Characteristics and size
  3. Classifying the microbes
  4. Importance of microbes
    1. Beneficial
    2. Harmful
  5. Biogenesis
  6. Isolation of bacteria from human skin
  7. Bacterial staining
    1. Meaning of bacteria
    2. Properties
    3. Classification
  8. Gram stain
    1. History and how it works
    2. Purposes
    3. Principle
  9. Indole production test
    1. Introduction
    2. Procedure
    3. Observation
  10. Methyl red test
    1. Procedure and result
  11. Voges-Proskaver test
    1. Aim and procedure
  12. Citrate utilization test
  13. Hydrogen sulphide production test
  14. Catalase test
  15. Blood group determination: Slide agglutination
    1. Human blood grouping
  16. Widal test
  17. Urine protein
  18. Heat test for protein
  19. Sulphosalicylic acid test
  20. Ketone bodies
  21. Nitroprusside test
  22. Urine glucose

What is microbiology? Why would any student enroll in microbiology? Now that you were told to take microbiology or now that you selected microbiology; it is important that you know what to expect. Microbiology is the study of science of (-logy) small (micro) life (bios). Biology was first used in 1802 and termed the "study of life." Small living organisms include animals like ticks, flukes, and lice; plants like pondweed or Lemna; fungi like ringworm and yeast; protists including Ameba, Paramecium, and many algae; bacteria which include E. coli, Streptococcus, and Staphylococcus; and viruses.

We must remember that microorganisms are living and share the characteristics associated with all living organisms including humans like yourself. Just think of a microbe as a "small person;" and then you will remember the characteristics of life or living organisms. Living organisms are composed of one or many cells; they carry on or perform cell metabolism or chemical activity; microbes grow, often increasing in size; microbes respond to the environment and any stimuli that might be present and or change; and also microbes reproduce. It is often during this reproductive process when microbes are most vulnerable and can be controlled.

[...] Sulphosalicylic Acid Test Principle Sulphosalicylic acid solution precipitates any protein in the urine specimen irrespective of the type albumin or Bence Jones. It is an anion precipitate that works by the neutralization of the protein cation. This method is more sensitive and reliable than the heat method. It can detect 5 to 10 mg/dL of protein in urine specimen. Reagent Sulphosalicylic acid; dissolve in distilled water in a beaker, and pour quantitatively into a 1-litre volumetric flask. Dilute to 1000ml with distilled or deionized water. [...]


[...] URINE PROTEIN Clinical Significance One of the important tests in routine urine analysis is to detect the presence of protein. Normal urine contains only traces of protein (albumin) and increased discharge of albumin in urine (referred to as ?proteinuria? urine may be correlated with findings of casts in the urinary sediment as part of their eventual diagnosis. In cases in the urinary sediment as part of the eventual diagnosis. In cases of renal disease, it is essential that the diagnosis be made and treatment started as soon as possible to prevent extensive and permanent renal damage. [...]


[...] The ABO and Rh are the major, clinically significant and the most important of all the blood group systems All people of ABO system can be divided into 4 major groups in this system and they are AB and O groups. This depends on the reactions obtained by mixing their red blood cells with two different reagents, known as Anti-A and Anti B for example in cell grouping by using known Anti A and Anti B the result obtained are as follows. [...]

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