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Study of Gibberellins’ Effect on Height and Biomass of Rosette and Wild Type Brassica rapa

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  1. Abstract
  2. The use of gibberellins or gibberellic acid
  3. The lab procedure and materials
  4. A two-way ANOVA
  5. Final height of Brassica rapa in response to concentrations
  6. Comparison between the average height of the control wild type Brassica rapa
  7. Literature cited

Gibberellins (GA) are plant hormones involved with overall plant growth. Brassica Rapa, a flowering plant species, which possesses the wild type phenotype or the rosette, dwarfed, phenotype, was used to explore the effects of Gibberellins on the two varieties' overall height, which can have an application in commercial farming for obtaining larger yields. The GA receptors are physiologically active and the levels of GA are causing the dwarfed appearance in the rosette variety. After seven days of plant growth, varying amounts of hormone were applied to the plants, ranging from 0.0ppm or 0.0 mg/ml GA to 1400ppm or 0.7mg/ml GA. After eighteen days of plant growth, the plants were measured for height and biomass. Data was inconclusive in determining if the GA had any effect on height, which does not necessarily mean GA has no effect, rather something potentially went askew in the experiment. The experiment should be modified and retested in order to find data that is more conclusive.

[...] We hope to see that both phenotypes of plants will show increased growth for height and biomass based on the concentration of GA received, also with adequate amounts of the GA, the rosette seeds will grow to a wild type height. Methods and Materials The lab procedure and materials used are similar to that detailed in Planting and care of Brassica rapa for Plant Hormone Experiment by Olsen et al. (2009); however, we used four watering systems with a total of ninety- six plants (forty-eight of each wild type and rosette variety Brassica rapa). [...]

[...] The average height of the wild type control and the average height of the 0.5 mg/ml rosette showed no significance other than the fact that with GA the rosette was able to obtain a height similar to that of the control wild type. More testing needs to be done to statistically reinforce this notion however. If we were to perform this experiment again, several key components could be modified to improve the experiment. We could try performing this experiment with different plants. [...]

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