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The Effect of Ampicillin Amounts and Incubation Time on E. Coli Growth

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  1. Introduction
    1. Antibiotic resistance
    2. E coli
    3. Resistance to bacteria
  2. Materials and methods
  3. Results
  4. Discussion
  5. References

Penicillin, derived from the soil mold Penicillium, was the primary antibiotic used to cure bacterial infections after being discovered in 1928 by Alexander Fleming. Briefly following its discovery and mass production bacteria began expressing resistance to this Nobel Prize winning medical breakthrough. Bacteria causing pneumonia, toxic shock syndrome, and gonorrhea were among many of the penicillin-resistant infections that arose over time and ultimately led to death. (Lewis)

[...] Using sterile technique, a small sample of 0.1 ml of cells were transferred from each of the six culture tubes to one Petri dish with ampicillin and one Petri dish with no ampicillin. Sterile beads were added to each Petri dish to assist in spreading out the bacteria cells by sliding the dishes around causing the beads to somewhat evenly distribute the cells. All twelve Petri dishes were then placed in an incubator for 21 hours at C to encourage growth of bacterial colonies in the dishes. [...]

[...] These among other errors may have had an effect on the outcome of the results. After performing this experiment and drawing conclusions we are able to propose new questions and ideas to research and investigate. We observed how E. coli was able to reproduce under specified conditions; however it would be interesting to see how additional types of bacteria would reproduce under the same conditions. Altering the bacteria used is one approach; another possibility would be to use an alternative antibiotic to replace the ampicillin to determine if bacteria were more or less receptive to other forms of antibiotics. [...]

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