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Gas Chromatography

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  1. Introduction
  2. Procedure
  3. Results
  4. Discussion
  5. Conclusion
  6. References

Chromatography is a technique used to separate and analyze chemicals. It was initially discovered in the early 1900's by chemist Mikhail Tsvett who was researching chlorophyll to separate plant pigments.1 The components undergoing separation are divided into two phases, a stationary phase, and a mobile phase. The stationary phase does not move while the mobile phase, which includes the sample, passes through the stationary phase. Different chemicals are impeded at different rates through the stationary phase based on individual properties which make them unique.2

[...] There are also less intermolecular forces in Freon 22, as evidenced by the lower boiling point when compared to Freon 123. The shorter retention time directly affects the ban width which was shorter for Freon 22 because the less time the component spends in the column the less time there is for interactions to take place between the Freon 22 and the stationary phase which generates a shorter ban width. Gas chromatography requires a carrier gas, a column, and a detector. [...]


[...] Results The gas flow rate (linear gas velocity) for our homemade chromatograph was calculated using the formula ? = where ? is the average linear gas velocity (in centimeters/second), L is the length of the gas chromatograph column (in centimeters), and tA is the average retention time for air (in seconds) ? = 29.5 cm / 4s Linear gas velocity = ? = 7.4 cm/s 11 For each halocarbon the green-blue flame appeared, intensified, and disappeared as portrayed in Figure 1. [...]

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