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Exploiting the use of fluorophore in extraction and direct detection of rice’s lipase activity

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Student
Level
General public
Study
biology
School/University
Monash...

About the document

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documents in English
Format
Word
Type
case study
Pages
5 pages
Level
General public
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  1. Introduction
  2. Extraction of Rice Proteins
  3. Materials and methods
  4. Conclusion

Lipase is the major protein in rice that catalyzes endogenous hydrolysis of lipids. Study on lipase activity is crucial in rice bran utilization, as it causes rancidity, severely affects the product quality and tarnishes its economic value. This experiment aimed to isolate lipase from different rice lines' tissues, detect its activity and determine its molecular weight. Proteins were extracted from grains of Bd192, Kasalath and 2514 rice lines by grinding, buffering and centrifuging. Extracted proteins were separated with SDS-PAGE and lipase activity was detected using 4-MU Butyrate.

Protein bands were visualized with Coomassie staining and molecular weight of lipase was determined by comparison with protein ladder. Bright UV bands were observed in all leaves but not grains samples. The molecular weights of lipase was approximately 36.7kDa. In conclusion, lipase was present in leaves samples of all rice lines but not in grains.

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