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Bioreactor: Optimizing E.Coli fermentation for Bacteria directed enzyme prodrug therapy

About the author

non
Level
Advanced
Study
others
School/University
CWU

About the document

Published date
Language
documents in English
Format
Word
Type
term papers
Pages
10 pages
Level
Advanced
Accessed
7 times
Validated by
Committee Oboolo.com
  1. Introduction
  2. Design of the study
    1. Title of the study
    2. Objectives of the study
    3. Scope of the study
  3. Methodology of the study
    1. Research design of the study
  4. Sources of data
    1. Primary source
    2. Secondary source
  5. Limitations of the study
  6. Theoritical background of the study
    1. Causes of stress in the workplace
    2. Job stress and health
  7. Work/life conflicts
  8. Eliminating barriers to advancement
    1. Preparing women for management
    2. Accommodating families
    3. Work-life practice guidelines
  9. Brief of companies policies and facilities
    1. Infosys
    2. Wipro
    3. SAP
    4. IBM
    5. Google
  10. Findings of the study
  11. Recommendations
  12. Bibliography

E.coli strain WM04 was determined as a viable option for Bacteria Directed Enzyme Prodrug Therapy. Determination of suitability for animal testing depends on sufficient quantities of bacteria being produced through fermentation. Optimal operating conditions are found for the production of WM04 by observing two important production variables: the quantity of bacteria produced and the capacity for survival of the bacteria.

One limitation to bacterial growth is the amount of oxygen supply. The concentration of dissolved oxygen and oxygen transfer rates are correlated to cell growth. Impellor velocity, ranging from 200-1000 rpm, allowed us to determine the optimal speed at which the greatest oxygen transfer rate was achieved in the bioreactor solution with minimal bacterial shearing.

The dissolved oxygen content of the media in the bioreactor was measured with an oxygen probe at varying impellor speeds. BioCommand Lite software was used to plot the dissolved oxygen concentration against the time of the run and the impellor speed versus time.

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