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Mapping of Drosophila Melanogaster incomplete gene

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documents in English
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30 pages
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  1. Introduction
  2. Experimental Plan, Observations and Analysis
  3. Dihybrid cross to determine linkage on chromosome
  4. Discussion
  5. Conclusion

Gene mapping is the process of assigning gene to specific chromosomes or specific loci on that chromosome. It also determines the relative distance between genes linked on the same chromosome (Strachan & Read 2004). Gene mapping is used to construct linkage map that shows the relative locus of all genes on each chromosome. This allows discovery of genetic markers or DNA sequences that are readily identifiable under microscope observation. By doing so, locations of critical genetic elements that are responsible for a disease could be identified. In humans, information of inheritance pattern and disease system obtained solely from pedigree analysis are limited, as sufficient progeny are not available to give reliable confirmation on characteristics and inheritance mode of disease-causing genes. Missing individuals or erroneous information in the pedigree further hinders analysis. Modern genetic linkage are particularly useful, as it can be examined in collaboration with pedigree to maximize information that can be collected to infer likely genotypes of any missing individuals.

According to Mendel's principle of independent assortment, pairs of alleles for a gene are assorted independently into gametes during meiosis, independent of other genes. This means that the behavior of one allele on one chromosome is unaffected by that of another allele on different chromosomes, giving a typical 9:3:3:1 phenotypic ratio for dihybrid crosses. However, it only considers segregation of genes located on different chromosomes.

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